Technologies

Agriculture

1. RT-PCR based Bt-Gene quantification assay & Kit

  • A cost effective RT-PCR based quantification of Bt-gene with high sensitivity and specificity

    Background
    With increasing number and complexity of GM events (single as well as stacked), testing for every GM event has become labour-intensive and costly. A GM event with single trait can be tested using a simple method, whereas identification and quantification of multiple or stacked traits or GM events require use of combination of high-throughput technologies. PCR, involving amplification of transgenic elements, is a widely employed method for GM detection. PCR-based GM detection methods are categorized on the basis of the level of specificity.

    Technology
    Gujarat Biotechnology Research Centre has developed a RT-PCR based diagnostic assay for Bt-gene expression detection using specific primers. The primers used has high sensitivity and specificity. The diagnostic assay is expected to be cost effective compared to presently available presence absence based quantification assays.

    Potential Applications
    Detection of Bt-gene expression using RT-PCR for copy number and field level expression studies, zygosity testing for breeding companies, Real-time PCR/qPCR assays have become the tool of choice for the rapid and sensitive determination and quantitation of cry gene copy number. Real-Time PCR allows for direct measurement of the amount of the BT gene. Determination of percent GMO food content important for import / export regulations.

    Value Proposition

    • Determination of percent GMO food content important for import / export regulations.
    • Highly specific and sensitive
    • Ability to differentiate the GM cotton in seed level
    • Cost effective and fast in comparison to existing end point techniques.

    Technology Status
    Assay has been tested in multiple Bt-cotton accessions and validated



2. Liquid Bio-fertilizer for Eco-Friendly and Sustainable Agriculture

  • A cost effective Bio-fertilizer for field applications

    Background
    Bio-fertilizers are artificially multiplied cultures of certain soil organisms that can improve soil fertility and crop productivity. Bio-fertilizers can be categorized into three major classes:

    • Nitrogen-Fixation
    • Phosphorus-Solubilizing
    • Potash -Solubiliser

    Bio-fertilizer are used for enhancing the productivity of the soil. They fix atmospheric nitrogen and solubilize phosphorus. Also they stimulate plant growth through synthesis of plant growth promoting substances.
    Liquid Bio-fertilizers have a distinct advantage in terms of cost saving on chemical fertilizers in addition to yield advantage. Chemical fertilizers otherwise may have negative effects on soil as well as human health, change the soil chemistry and these soils no longer support plant growth in the long run.

    Technology
    Gujarat Biotechnology Research Centre has developed liquid bio-fertilizers from strains of Bank-A-Bug repository and screened them for Nitrogen-Fixation, Phosphorus-Solubilizing, Potash –Solubilising properties.

    Potential Applications
    There are three ways of using Liquid Bio-fertilizers

    • Seed treatment
    • Root dipping
    • Soil application

    Benefits
    Assay has been tested in multiple Bt-cotton accessions and validated

    • Eco Friendly
    • Effective and Efficient used in organic farming
    • Shelf life minimum 1 year
    • Easy use and transport
    • Suitable for drip irrigation
    • Suitable for Green house technology

    Technology Status
    Technology is ready and can be packed in different volumes and combinations as per requirement


3. Eco-friendly Sustained Release Nitrogen Biofertilizer

  • A effective Sustained release biofertilizer

    Background
    Inoculation of Plant for non-symbiotic rhizosphere bacteria for crop improvement is complicated task. A slow-release fertilizer releases nutrients to plants slowly over some time. Slow-release fertilizers are usually dry blends or granular formulas, they are easy to spread and are suitable for covering broad areas.
    Sustained release fertilizer has a distinct advantage in terms of cost saving on chemical fertilizers and liquid biofertilizer in addition to yield advantage. Chemical slow release fertilizers otherwise may have negative effects on soil and change the soil chemistry and these soils no longer support plant growth in the long run and these limitations of chemical slow release fertiliser and liquid biofertilizer led to exploration of sustained release biofertilizer
    Slow-release fertilizers release their nutrients slowly after each irrigation. The benefits to the farmer are that the plants don't receive toxic amounts of fertilizers all at once.

    Technology
    Gujarat Biotechnology Research Centre has developed Bead trapped with bacterial culture as Sustained release fertilizer by screening of different Strains of Nitrogen fixer for its Nitrogen fixing ability from Bank a Bug repository. Complete technology for the production is based on immobilization of Nitrogen fixing bacteria in a matrix which is ecofriendly and helps in sustained release of bacteria in soil thus, rendering fertility for longer period.

    Potential Applications
    This can be used in following ways

    • Sowing with Seed
    • Spreading on surface of turfs and lawns
    • With roots of plants

    Advantages

    • Prevents Volatilization, Denitrification, Leaching and Run off of Nitrogen from Soil
    • No problem of toxic residue
    • long persisting effect
    • Conventional technique of application

    Technology Status
    The processes are developed and can be packed bulk on requirement


4. Mycorrhizal Bio-fertilizer

  • A cost effective bio-fertilizer for promoting growth and enhanced nutrient uptake especially phosphorous

    Brief Description
    India consumes about 17.8 million tonnes of fertilizer per annum to meet the food security requirements. Overuse of fertilizers has led to nutrient leaching, environmental and human health problems, from climate change and dead zones in the oceans to cancer and reproductive risk. Agrarian economy is also suffering. The need of the hour is to switch to cost effective, environment friendly bio fertilizers to prevent further damage.

    Technology
    Gujarat Biotechnology Research Centre has developed a mycorrhizal biofertilizer which contains ample infective propagules that can result in the production of a plant of great vigor. The mycorrhizal biofertilizer helps in establishing a mutualistic association between plants and many of fungi from the phylum Glomeromycota. Upon colonizing the roots, arbuscular mycorrhizal fungi (AMF) gives rise to hypha which act as vehicle for nutritional exchange aiding in mobilizing uptake of phosphorous (beyond depleted zone), micronutrients and other untapped nutrition in the soil. It will help in improving soil texture and structure and increase resistance against diseases, drought, salinity, heavy metals and other abiotic stresses. It will also help in withstanding transplant shock during hardening of tissue cultured raised plants.
    As soon as the plant environment changes from sterile compost to soil, the root environment changes radically which can lead to plant failure and poor growth. In most soils, there are not enough of native mycorrhizal fungi close to the new plants roots to colonize fast enough to achieve the benefits of complete colonization. A new plant with fully functioning mycorrhiza root system will be most rewarding organic system

    Problem Addressed
    India consumes about 17.8 million tonnes of fertilizer per annum to meet the food security requirements. Overuse of fertilizers has led to nutrient leaching, environmental and human health problems, from climate change and dead zones in the oceans to cancer and reproductive risk. Agrarian economy is also suffering. The need of the hour is to switch to cost effective, environment friendly bio fertilizers to prevent further damage.
    As soon as the plant environment changes from sterile compost to soil, the root environment changes radically which can lead to plant failure and poor growth.
    In most soils, there are not enough of native mycorrhizal fungi close to the new plants roots to colonize fast enough to achieve the benefits of complete colonization.
    A new plant with a fully functioning mycorrhizal root system will be the most rewarding and symbiotic association will lead to reduction of chemical fertilizer and pesticide inputs.

    Potential Applications
    It is suitable for organic farming and commercial agricultural practices.

    Method of Application
    Soil Application: Mix 2 to 4 Kg of Mycorrhizal bio-fertilizer with 100 Kgs of Organic Manure/vermi compost or dried farm yard manure and broadcast in the main field just before sowing / transplanting or apply in root zone of standing crop by row or spot or broadcasting method (50 g / plant). This method can also be used for lawn making.
    Drip/drench Irrigation: Mix 2 kg mycorrhizal biofertilizer with sufficient quantity of water and apply in one-acre soil rhizosphere by drench or drip application.
    Nursery application: The inoculum (20gm/plant) can be applied at 2-3 cm below the soil level at the time of sowing.
    Polybag seedlings (10 kg of mycorrhizal biofertilizer for 1000 kg of potting mixture)

    USP/Advantages

    • Cost effective as compared to existing mycorrhizal bio-fertilizer.
    • Effective for wide spectrum of agricultural/ forestry crop
    • User and environment friendly

    IP status
    Available for licensing

    Technology Status
    Bio fertilizer will be