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Sep 02, 2023 , Hands-on Training Program on "Plant Tissue Culture & Transgenics".
Hands-on Training Program on "Plant Tissue Culture & Transgenics".
Jointly organized by Gujarat Biotechnology Research Centre (GBRC) and Anand Agricultural University (AAU), Anand.
Training from 9th to 21st October, 2023
Last Date of Registration - 23rd September, 2023
Registration link - https://io.gbrc.res.in/ptc
Day-1: Inauguration ceremony for training and Introduction to media components, types of tissue culture techniques, media preparation
Day-2: New culture initiation: Explant screening, cutting of explant, sterilization and inoculation
Day-3: Primer designing for gene isolation, Competent cells preparation
Day-4 & 5: Construct preparation for vectors, restriction digestion and ligation and confirmation by PCR
Day-6: Transformation of recombinant vectors in competent cells and selection
Day-7: Selection of positive colonies, Plasmid isolation and transformation in agrobacterium
Day-8: Agrobacterium mediated plant transformation
Day-9: Gene gun-mediated plant transformation
Day-10: In planta transformation by agrobacterium
Day-11: Selection and analysis of transformants and valedictory ceremony
Hands-on Training Program on "Plant Tissue Culture & Transgenics".